what is the first event to take place in translation in eukaryotic cell?

what is the first event to take place in translation in eukaryotic cell?

a. An clongation of the polypeptide chain by the addition of amino acids

b. Binding of the larger ribosomal subunits to the smaller ribosomal subunits

c. Base pairing of activated methionine-tRNA to AUG of messenger RNA

d. The small subunits of ribosome recognizing and attaching to 5′ cap of mRNA

Answer: c) Base pairing of activated methionine-tRNA to AUG of messenger RNA

In a eukaryotic cell, the first event that occurs in translation is base pairing of activated methionine-tRNA to AUG start codon on mRNA.

This is done after the two ribosomal subunits have been assembled on mRNA. The methionine-tRNA is recruited and positioned by the small subunit of the ribosome such that its anticodon region can pair correctly with an AUG start codon. This first linking serves for proper mRNA alignment in the ribosome acclimating to its proper starting point.

It is only after this initiator codon recognition and base pairing that elongation follows, resulting in the addition of another amino acid on its tRNA . Therefore, the association of methionine-tRNA with AUG represents a crucial initial reaction in translation and guarantees precision during gene expression.