What is the function of the microscope diaphragm?
a. source of light
b. condenses light from the light source into a narrow beam
c. regulates the amount of light passing through the specimen
d. used to focus the specimen
e. holds the objective lenses
Answer: c. regulates the amount of light passing through the specimen
The microscope diaphragm is also called the iris diaphragm or aperture. It is very useful in regulating the amount of light that is allowed to pass through the specimen analysed. It has metalwork that can be slid open or closed to a certain extent, with this extremity being variable. Macroscopics can also manipulate the diaphragm to focus the illumination on the specimen producing high contrast and clarity of the image. This is particularly useful when looking at specimens of different thicknesses or optical characteristics. The diaphragm should be adjusted in a way to allows the light to equalize the brightness of the field and also minimize glare while at the same time offering a good visualization of the microscopic image. It is almost indispensable, for obtaining the most effective visualization of the microstructures of different kinds of specimens.
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